This study was carried out to gather basic data for the purpose of proposing a plan to improve the water quality and conserve the aquatic ecosystem of the Singu Agricultural Reservoir in Korea. The water quality, sediment composition, benthic macroinvertebrate distribution, and fish distribution in the Singu Reservoir were analyzed; the reservoir is located close to farmlands, forests, villages, and livestock breeding areas. The results of the water quality analysis are as follows: 5.8~7.8 mg/L for dissolved oxygen, 13.1~20.7 mg/L for chemical oxygen demand, 14.4~18.8 mg/L for suspended solid, 0.96~1.70 mg/L for total nitrogen, 0.07~0.11 mg/L for total phosphorous, and 41.9~49.8 μg/L for chlorophyll a. In total, 75 benthic macroinvertebrate specimens belonging to 4 classes, 7 orders, 14 families, and 17 species were recorded. The ecological scores of the benthic macroinvertebrate communities ranged from 11 to 23. Fish specimens recorded belonged to two families and four species. The dominant fish species were Carassius auratus and Pseudorasbora parva, both of which are tolerant to several water-pollutants.