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ISSN : 1225-4517(Print)
ISSN : 2287-3503(Online)
Journal of Environmental Science International Vol.28 No.1 pp.75-84
DOI : https://doi.org/10.5322/JESI.2019.28.1.75

Cloning and Sequencing of the Mitochondrial Cytochrome c Oxidase Subunit Ⅱ Gene from Rhabditidae Family Nematode

Sang Mong Lee,Hong Joo Son,Keun Ki Kim,Chang Oh Hong,Hyean Cheal Park*
Department of Life Science & Environmental Biochemistry, Pusan National University, Miryang 50463, Korea
*Corresponding author: Hyean Cheal Park, Department of Life Science & Environmental Biochemistry, Pusan National University, Miryang 50463, Korea Phone : +82-55-350-5547

Abstract

Cytochrome c oxidase subunit Ⅱ gene(COⅡ gene) is subunit of cytochrome oxidase, which is complex Ⅳ of mitochondria electron transport system. It has been frequently used in molecular phylogenetic studies because the speed of its DNA variation is faster than that of nucleus. It is especially useful in phylogenetic study of molecular biology in insects. In this study, we cloned and sequenced COⅡ gene of mitochondria DNA from Rhabditidae family nematode. Our results showed that this gene is comprised of 696 base pairs(bp). In the analysis of similarity of this gene with other known genes of 14 species of nematodes in Rhabditida order, we identified that this gene has high similarity with that of Caenorhabditis briggsae(86.0%) and C. elegans(85.6%) in Rhabditidae family. On the meanwhile, it has very low similarity with that of Angiostrongylus cantonensis(31.8%) in Angiostrongylidae family and Metastrongylus salmi(31.6%) in Metastrongylidae family. Based on the results of this study, we suggest that this nematode is closely related with that of Caenorhabditis genus in Rhabditidae family.

Rhabditidae과 선충의 COⅡ 유전자 클로닝 및 염기서열 분석

이상몽,손홍주,김근기,홍창오,박현철*
부산대학교 생명환경화학과

초록

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